| 质谱法分析大鼠精子发生过程中组蛋白H1变异体的多样性 |
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| 引用本文： | 鲁爽,程露阳,汪卓群,李欣,石心泉,贾孟春,马旭. 质谱法分析大鼠精子发生过程中组蛋白H1变异体的多样性[J]. 中国实验动物学报, 2009, 17(5): 335-340 |
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| 作者姓名： | 鲁爽 程露阳 汪卓群 李欣 石心泉 贾孟春 马旭 |
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| 作者单位： | 1. 北京协和医学院研究生院,北京,100085;国家人口计生委科学技术研究所,北京,100081
2. 国家人口计生委科学技术研究所,北京,100081 |
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| 基金项目： | 国家"973"基础研究基金 |
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| 摘 要： | 目的为探究连接组蛋白H1在精子发生过程染色体重构中的功能,了解一共有多少种连接组蛋白H1参与各期生精细胞的染色体的构建。方法分离高纯度的SD大鼠的各期生精细胞,提取组蛋白,应用SDS-PAGE分离组蛋白的各组分,组蛋白（H1）经过蛋白酶（Glu-c和Arg-c）酶切,应用质谱进行检测。结果鉴定了组蛋白H1的体细胞亚型（H1.1-H1.5）和睾丸特异的连接组蛋白亚型（H1t）。组蛋白H1t分别表达在精原细胞,精母细胞和圆形精子细胞中。结论大鼠精子发生过程中,其主要连接组蛋白H1的种类是：H1.1-H1.5和H1t。
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| 关 键 词： | 精子发生 组蛋白H1的亚型种类 液质联用 |
Mapping of Linker Histone H1 Variants That Participate in Rat Spermatogenesis |
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| LU Shuang,CHENG Lu-Yang,WANG Zuo-Qun,LI Xin,SHI Xing-quan,JIA Meng-Chun,MA Xu. Mapping of Linker Histone H1 Variants That Participate in Rat Spermatogenesis[J]. Acta Laboratorium Animalis Scientia Sinica, 2009, 17(5): 335-340 |
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| Authors: | LU Shuang CHENG Lu-Yang WANG Zuo-Qun LI Xin SHI Xing-quan JIA Meng-Chun MA Xu |
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| Affiliation: | 1. Graduate School, Peking Union Medical College, Beijing 100085, China; 2. Department of Genetics, National Research Institute for Family Planning, Beijing 100081 ) |
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| Abstract: | Objective In order to understand the global dynamics of chromatin structure and function during spermatogenesis,the histone H1 variants incorporated into nucleosome were analyzed.Methods Histons were extracted from the spermatogonia,spermatocytes and round spermatids of Sprague-Dawley rats,separated by high performance liquid chromatography,and identified by enzymatic digestion（Glu-c and Arg-c） and LC-MS/MS.Results Somatic subtypes（H1.1-H1.5） and a testis-specific linker histone（H1t） were identified.H1t was observed in the spermatogonia,spermatocytes and round spermatids.Conclusion The chromatin linker histones H1.1-H1.5 and H1t participate in the genesis of spermatogenic cells. |
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| Keywords: | Spermatogenesis Histone H1 variants LC-MS/MS |
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