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Identification of zeatin and zeatin riboside in cones of the hop plant and their possible role in cone growth
Authors:Watanabe  Naoharu; Yokota  Takao; Takahashi  Nobutaka
Institution:Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo, Japan
Abstract:Cytokinin contents of fertilized cones, unfertilized cones andseeds of the hop plant (Humulus lupulus L.) were found to be0.08 ppm (Benzyladenine equivalent), 0.03 ppm and 0.5 ppm respectively.Major cytokinin in the former two was determined to be zeatinriboside based on gas chromatography-mass spectrometry afterseveral purification steps. Two more cytokinins were detectedin fertilized cones, one of which was determined to be zeatin.The possible role of endogenous cytokinin in cone growth isdiscussed in terms of quantitative and qualitative data obtained.Furthermore, effective purification techniques introduced inthis work are also discussed. (Received December 2, 1977; )
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