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| Kit无义突变致W~(-3Bao)小鼠生殖腺异常及纯合子贫血死亡 | |
| 引用本文： | 吴培林,尹洪萍,殷黎静,朱洁,曾咏梅,刘桂杰,亢晓冬,俞利平,顾美儿,袁红,吴宝金. Kit无义突变致W~(-3Bao)小鼠生殖腺异常及纯合子贫血死亡[J]. 动物学研究, 2009, 30(1). DOI: 10.3724/SP.J.1141.2009.01045 |
| 作者姓名： | 吴培林 尹洪萍 殷黎静 朱洁 曾咏梅 刘桂杰 亢晓冬 俞利平 顾美儿 袁红 吴宝金 |
| 作者单位： | 1. 杭州师范大学实验动物科学实验室,浙江,杭州,310036 2. 扬州大学比较医学中心,江苏,扬州,225001 3. 杭州师范大学实验动物科学实验室,浙江,杭州,310036;扬州大学比较医学中心,江苏,扬州,225001 |
| 基金项目： | 国家自然科学基金面上项目 |
| 摘 要： | W-3Bao是本研究组通过诱变获得、Kit无义突变的白斑小鼠.突变基因杂合子小鼠腹部、四肢肢端及尾尖白化,其部分精曲小管内无精原细胞.突变纯合子小鼠在胚胎后期色泽苍白、个体矮小,于出生前后死亡;血液学检查发现纯合子小鼠血色素极低且红细胞变大:18.5天胚胎的连续切片可见精曲小管轮廓欠清晰,精原细胞分散分布于睾丸间质,未迁入精曲小管:卵巢结构紊乱,无明显的原始卵泡结构;骨髓等器官组织未见显著异常.结论:Kit无义突变不仅导致了W-3Bao杂合子小鼠白斑形成及纯合子小鼠贫血死亡,同时影响生殖腺发育. |
| 关 键 词： | W-3Bao小鼠 Kit基因 死亡 大红细胞贫血 生殖腺异常 |
Gonadial Abnormality and Homozygous Decease from the Nonsense Mutation of Kit in W~(-3Bao) Mouse |
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| WU Pei-lin,YIN Hong-ping,YIN Li-jing,ZHU Jie,ZENG Yong-mei,LIU Gui-jie,KANG Xiao-dong,YU Li-ping,GU Mei-er,YUAN Hong,WU Bao-jin. Gonadial Abnormality and Homozygous Decease from the Nonsense Mutation of Kit in W~(-3Bao) Mouse[J]. Zoological Research, 2009, 30(1). DOI: 10.3724/SP.J.1141.2009.01045 | |
| Authors: | WU Pei-lin YIN Hong-ping YIN Li-jing ZHU Jie ZENG Yong-mei LIU Gui-jie KANG Xiao-dong YU Li-ping GU Mei-er YUAN Hong WU Bao-jin |
| Affiliation: | 1. Laboratory of Experimental Animal Science;Hangzhou Normal University;Hangzhou 310036;2. Comparative Medicine Center of Yangzhou University;Yangzhou 225009 |
| Abstract: | The W-3Bao mouse, which was obtained in previous ENU mutagenesis project, is a new mutant caused by the nonsense mutation of Kit gene. Mating and gross observing combining with PCR and sequencing were used for determining the genotypes of the W-3Bao mice. Embryonic development, hematological detection and histopathologic section methods were used for their phenotype analysis. The results showed that the W-3Bao/+ mouse was white belly, white limb terminals and white tail tip. However, there was no difference... |
| Keywords: | W-3Bao mouse Kit gene Decease Macrocytic anemia Gonadial abnormality |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |