Analysis of the genetic diversity of Bemisia tabaci populations in Shanxi province, China |
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Authors: | Gui-Yun Zhang Li-Ping Zhang You-Jun Zhang Zhen Liu Hong-Jun Zhang Qiao-Lan Fan Ming-Feng Wei |
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Affiliation: | aCotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng 044000, China;bInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081,China;cInstitute for Control of Agrochemicals, Ministry of Agriculture, Beijing 100026, China |
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Abstract: | Five different primer combinations were used for the analysis of 152 B biotype Bemisia tabaci (Gennadius) individuals and five Trialeurodes vaporairiorum individuals collected from 19 counties and seven host plants in Shanxi province in China, respectively. The main objective of the present study was to use AFLP markers to determine the genetic diversity of B. tabaci populations collected from Shanxi Province. The use of these primer combinations allowed the identification of 127 polymorphic bands (52.26%) from 60 to 500 bp. The average number of polymorphic bands per primer was 25.4 while the range for the five primers was 20–32. The average degree of heterozygosity was 0.251, while the range for the five primers was 0.204–0.289. The results suggested definite genetic diversity among different B. tabaci populations. Cluster analysis showed that B. tabaci populations were firstly scattered to three genetic groups according to the regions, then every genetic group was scattered to several subgroups according to the host plants, which revealed the genetic variability of B biotype B. tabaci populations has been not only among different regions, but also among different host plants in Shanxi Province. |
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Keywords: | Bemisia tabaci (Gennadius) Genetic diversity AFLP marker Shanxi Province |
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