节杆菌K1108乙内酰脲酶产酶条件研究

研究了乙内酰脲酶产生菌节杆菌K1108的产酶条件。该菌乙内酰脲酶为诱导酶，存在于细胞内，乙内酰脲水解酶和N氨甲酰氨基酸水解酶是同时被诱导产生。最适诱导物为5苄基乙内酰脲，而5吲哚甲基乙内酰脲和5苯基乙内酰脲等不能诱导其酶的产生。筛选到一种安慰诱导物，诱导活性提高了2倍多。对产酶培养基进行了筛选和优化，在最适条件下，K1108产酶能力可达108U/mL。

Studies on Hydantoinase Producing Conditions of Arthrobacter K1108

The hydantoinase-producing conditions from strain Arthrobacter K1108 were investigated.It is shown that the hydantoinase in the strain is intracellular and inducible.Its optimal inducer is 5-benzylhydantoin,while 5-indolylmethylhydantoin and 5-phenylhydantoin cannot induce the production of hydantoinase in K1108.A gratuitous inducer was designed,with which the hydantoinase production is 343% as much as that with 5-benzylhydantoin.The media for culturing the bacteria were screened and optimized.Under optimal conditions,the specific activity of K1108 cells reached 10.8U/mL.