A mechanism for substrate-Induced formation of 6-hydroxyflavin mononucleotide catalyzed by C30A trimethylamine dehydrogenase |
| |
| Authors: | Lu Xingliang Nikolic Dejan Mitchell Deanna J van Breemen Richard B Mersfelder John A Hille Russ Silverman Richard B |
| |
| Institution: | Department of Chemistry, Northwestern University, Evanston, IL 60208-3113, USA. |
| |
| Abstract: | Experiments are described to determine the origin of the 6-hydroxyl group of 6-hydroxyFMN produced by the substrate-induced transformation of FMN in the C30A mutant of trimethylamine dehydrogenase. The conversion of FMN to 6-hydroxyFMN is carried out in the presence of H(2)(18)O and 18O(2), and the results clearly show that the 6-hydroxyl group is derived from molecular oxygen and not from water. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
