Isolation and characterization of eight microsatellite loci from the endangered plant species Hypochaeris salzmanniana (Asteraceae) |
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Authors: | Claudete F Ruas Thiago J Nakayama M Á Ortiz Mayra A Kuroki Tod F Stuessy Karin Tremetsberger Eduardo A Ruas Melissa de Oliveira Santos Salvador Talavera Paulo M Ruas |
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Institution: | 1. Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, 86051-990, Londrina, Paraná, Brazil 2. Departamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Apto-1095, 41080, Sevilla, Spain 3. Department of Systematic and Evolutionary Botany, Faculty Center of Botany, University of Vienna, Vienna, Austria 4. Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, 86051-990, Londrina, Paraná, Brazil 5. Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e Evolu??o, Universidade Estadual de Campinas, Bar?o Geraldo, CP 6010, Campinas, SP, CEP 13083-970, Brazil
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Abstract: | We report the isolation and characterization of eight microsatellite loci for Hypochaeris salzmanniana, an endangered species endemic to the southwestern coast of Spain and the Atlantic coast of Morocco. A total of 32 alleles
were detected across a sample of 45 individuals, with an average number of 4.0 alleles per locus. The average polymorphic
information content (PIC) was 0.533 and the observed (H
O) and expected (H
E) heterozygosity values varied from 0.022 to 0.978 and from 0.434 to 0.759, respectively. Five loci exhibited significant
deviation from Hardy–Weinberg equilibrium (P ≤ 0.001) and three pairs of loci showed significant linkage disequilibrium (P ≤ 0.01). The eight loci were tested for transferability in three others species (H. arachnoidea, H. glabra, and H. radicata) belonging to the same section of H. salzmanniana. With the exception of locus Hsalz7, all loci successfully amplified in the three species. These preliminary data confirm
the usefulness of microsatellite markers for assessing the ecology and genetic structure of H. salzmanniana and to understand the evolution of species within the section Hypochaeris. |
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