Triton X-100 alters the resistance level of methicillin-resistant Staphylococcus aureus to oxacillin |
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Authors: | H Komatsuzawa M Sugai C Shirai J Suzuki K Hiramatsu H Suginaka |
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Institution: | Department of Microbiology, Hiroshima University School of Dentistry, Hiroshima 734, Japan; Department of Internal Medicine II, Hiroshima University School of Medicine, Hiroshima 734, Japan; Department of Bacteriology, Juntendo University School of Medicine, Tokyo, Japan |
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Abstract: | Abstract We used population analysis to examine the effects of Triton X-100 on the level of resistance to oxacillin of 18 methicillin-resistant Staphylococcus aureus . In the presence of 0.02% Triton X-100, 17 formerly methicillin-resistiant strains exhibited enhanced sensitivity to oxacillin. One homogeneous isolate, KSAF1 was barely affected by the Triton X-100. Sensitivities of lysostaphin, 51 kDa N -acetylglucosaminidase and 62 kDa N -acetylmuramoyl-l-alanine amidase to heat-inactivated cells were not affected when the bacteria were grown in 0.02% Triton X-100. Our data, together with those of a previous study, suggested that Triton X-100 alters the resistance level of methicillin-resistant S. aureus by influencing a factor(s) other than PBPs, bacteriolytic enzymes, or femAB products. |
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Keywords: | Methicillin resistance Staphylococcus aureus Triton X-100 Population analysis Oxacillin |
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