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Purification of recombinant ribonuclease T1 expressed in Escherichia coli
Authors:B A Shirley  D V Laurents
Affiliation:Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843.
Abstract:A protocol for the rapid purification of ribonuclease T1 expressed from a chemically synthesized gene cloned into Escherichia coli is described. QAE ion-exchange and Sephadex G-50 chromatography are used to give over 300 mg (88% yield) of pure ribonuclease T1 from 61 of liquid culture in 3 days. We also report a new absorption coefficient for RNase T1: E1%278 nm = 15.4.
Keywords:
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