Purification of recombinant ribonuclease T1 expressed in Escherichia coli |
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Authors: | B A Shirley D V Laurents |
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Affiliation: | Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843. |
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Abstract: | A protocol for the rapid purification of ribonuclease T1 expressed from a chemically synthesized gene cloned into Escherichia coli is described. QAE ion-exchange and Sephadex G-50 chromatography are used to give over 300 mg (88% yield) of pure ribonuclease T1 from 61 of liquid culture in 3 days. We also report a new absorption coefficient for RNase T1: E1%278 nm = 15.4. |
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