Differentiation of spermatogonial stem cell-like cells from murine testicular tissue into haploid male germ cells in vitro |
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Authors: | Peng Wang Li-Juan Suo Hua Shang Ying Li Guang-Xuan Li Qing-Wang Li Jian-Hong Hu |
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Affiliation: | 1. College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China
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Abstract: | In vitro differentiation of spermatogonial stem cells (SSCs) promotes the understanding of the mechanism of spermatogenesis. The purpose of this study was to isolate spermatogonial stem cell-like cells from murine testicular tissue, which then were induced into haploid germ cells by retinoic acid (RA). The spermatogonial stem cell-like cells were purified and enriched by a two-step plating method based on different adherence velocities of SSCs and somatic cells. Cell colonies were present after culture in M1-medium for 3 days. Through alkaline phosphatase, RT-PCR and indirect immunofluorescence cell analysis, cell colonies were shown to be SSCs. Subsequently, cell colonies of SSCs were cultured in M2-medium containing RA for 2 days. Then the cell colonies of SSCs were again cultured in M1-medium for 6–8 days, RT-PCR and indirect immunofluorescence cell analysis were chosen to detect haploid male germ cells. It could be demonstrated that 10−7 mol l−1 of RA effectively induced the SSCs into haploid male germ cells in vitro. |
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Keywords: | Differentiation Spermatogonial stem cells (SSCs) Murine Retinoic acid |
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