Chemical relaxation studies on bovine serum albumin. I. Reactions of the imidazole groups at pH 6 to 8

In the pH range of 6 to 8, the proton transfer reactions of BSA were measured using the temperature jump relaxation technique. The rate data were compared to the results using imidazole itself to establish that the observed reactions were those of the imidazole groups in the BSA. Upon the addition of calcium and gadolinium to the BSA solutions, no metal ion complexation was observed for either cation at the imidazole sites.