Purification and characterization of carbonyl reductase from Geotrichum candidum |
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Authors: | Amit Singh Mani Shankar Bhattacharyya Uttam Chand Banerjee |
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Institution: | aDepartment of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar-160 062, India |
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Abstract: | Geotrichum candidum is well known for the reduction of prochiral ketones to chiral alcohol with high yield and excellent enantioselectivity. Carbonyl reductase from G. candidum was purified by ammonium sulphate precipitation, anion exchange and hydrophobic interaction chromatographies. Gel filtration chromatography together with SDS-PAGE revealed this protein to be a dimer of 60 kDa subunits. Maximum enzyme activity was found in acetate buffer at pH 5.4 with t1/2 of 7.13 h at 30 °C and t1/2 of 2.8 h at 65 °C. The enzyme was inhibited by p-hydroxymercuribenzoate and hydroxylamine indicating the involvement of thiol and carbonyl groups in the reduction reaction catalyzed by the enzyme. Chelating agents also reduced the enzyme activity indicating the requirement of metal ions as cofactors. The purified carbonyl reductase was found to be highly selective for ketones containing naphthyl ring, whereas aryl or hetero-aryl ketones showed very less or no activity at all. |
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Keywords: | Geotrichum candidum Carbonyl reductase S (− )-1-(1′ -naphthyl) ethanol Purification Characterization Thermostability |
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