Characterisation of the wheat phospholipid fraction in the presence of nickel and/or selenium |
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Authors: | Przemysław Bernat Ewa Gajewska Tadeusz Bernat Marzena Wielanek |
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Institution: | 1. Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of ?ód?, Banacha 12/16, 90-237, ?ód?, Poland 2. Department of Plant Physiology and Biochemistry, Faculty of Biology and Environmental Protection, University of ?ód?, Banacha 12/16, 90-237, ?ód?, Poland
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Abstract: | The influence of nickel (Ni) and/or selenium (Se) on phospholipid composition was studied in shoots and roots of wheat seedlings. Phospholipid differences between samples were analysed using liquid chromatography/electrospray ionization–MS coupled. A total of 39 lipid species were identified. Individual phospholipids were then quantified using a multiple reaction monitoring method. In the roots, Ni toxicity was associated with an elevated level of phosphatidic acid species. In the shoots, the phosphatidylcholine/phosphatidylethanolamine ratio was about fivefold higher than in roots and decreased in Ni-treated samples. Additionally, the concentrations of phospholipid species containing C 18:3 fatty acid were reduced. Lipidome data were then analyzed using principal component analysis, which confirmed the compositional changes in phospholipids in response to Ni and Ni + Se. In contrast, the phospholipid profiles of wheat seedlings exposed to Se alone showed more similarities with the control. Together, our results suggested that the presence of Se, despite a considerable improvement of growth of Ni-treated wheat, did not counterbalance negative effect of Ni on the phospholipid composition in wheat roots and shoots. |
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