A standard procedure for the micropropagation of the neem tree (Azadirachta indica A. Juss) |
| |
Authors: | J P Eeswara T Stuchbury E J Allan A J Mordue |
| |
Institution: | (1) Department of Agriculture, University of Aberdeen, 581 King Street, Aberdeen, AB24 5UA, UK Fax no.: +44-1224-273731 E-mail: t.stuchbury@abdn.ac.uk, GB;(2) Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB9 2TN, UK, GB |
| |
Abstract: | Micropropagated shoots were initiated from leaf explants of the neem tree, Azadirachta indica A. Juss. Regardless of their origin, shoots were successfully produced by culturing leaf explants on Murashige and Skoog
medium containing benzylaminopurine (1 mg l–1), kinetin (0.8 mg l–1) and adenine sulphate (6 mg l–1) in complete darkness. These shoots were further multiplied on Murashige and Skoog medium containing benzylaminopurine (0.1
mg l–1), kinetin (0.08 g l–l) and adenine sulphate (0.6 mg l–1). Within 32 weeks, 80 shoots could be produced from a single leaf explant (10 mm×10 mm). Fifty-five percent of these shoots
rooted on Murashige and Skoog medium containing indolebutyric acid (1 mg l–1) and all of these grew on transfer to soil.
Received: 5 May 1996 / Revision received: 23 August 1996 / Accepted: 5 October 1996 |
| |
Keywords: | Neem Azadirachta indica Micropropagation Shoot multiplication |
本文献已被 SpringerLink 等数据库收录! |
|