Development of an effective sample preparation method for the proteome analysis of body fluids using 2-D gel electrophoresis |
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Authors: | Joo Won-A Lee Do-Youn Kim Chan-Wha |
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Institution: | Graduate School of Life Sciences and Biotechnology, Korea University, Seoul. |
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Abstract: | A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained. |
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