Ectodermal P2X receptor function plays a pivotal role in craniofacial development of the zebrafish |
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Authors: | Sarah Kucenas Jane A Cox Florentina Soto Angela LaMora Mark M Voigt |
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Institution: | (1) Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, 1402 S. Grand Blvd, St. Louis, MO 63104, USA;(2) Present address: Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA;(3) Present address: Department of Biological Structure, University of Washington, Seattle, WA 98195, USA |
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Abstract: | P2X receptors are non-selective cation channels operated by extracellular ATP. Currently, little is known concerning the functions
of these receptors during development. Previous work from our lab has shown that zebrafish have two paralogs of the mammalian
P2X3 receptor subunit. One paralog, p2rx3.1, is expressed in subpopulations of neural and ectodermal cells in the embryonic head. To investigate the role of this subunit
in early cranial development, we utilized morpholino oligonucleotides to disrupt its translation. Loss of this subunit resulted
in craniofacial defects that included malformation of the pharyngeal skeleton. During formation of these structures, there
was a marked increase in cell death within the branchial arches. In addition, the epibranchial (facial, glossopharyngeal,
and vagal) cranial sensory ganglia and their circuits were perturbed. These data suggest that p2rx3.1 function in ectodermal cells is involved in purinergic signaling essential for proper craniofacial development and sensory
circuit formation in the embryonic and larval zebrafish. |
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Keywords: | P2X Zebrafish Morpholino Chondrogenesis Sensory ganglia |
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