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Role of C-terminal Cys-rich Region of Phytochelatin Synthase in Tolerance to Cadmium Ion Toxicity |
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| Authors: | Sachiko Matsumoto Mun’delanji Vestergaard Takafumi Konishi Shingo Nishikori Kentaro Shiraki Naoki Tsuji Kazumasa Hirata Masahiro Takagi |
| Institution: | 1. School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi City, Ishikawa, 923-1292, Japan 2. Division of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto, 8620976, Japan 3. Institute of Applied Physics, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8573, Japan 4. Department of Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka, 565-0871, Japan |
| Abstract: | Phytochelatins (PCs) are Cys-rich peptides, synthesized by PC synthase in response to heavy metal ions. The C-terminal Cys-rich region of the PC synthase has homology with functional domains of metallochaperone, metallothionein and thioredoxin. To test the possibility that the C-terminal Cys-rich region of PC synthase has a role in regulating PC synthesis, we introduced point mutations into the PC synthase, replacing Cys358, Cys359 Cys363 and Cys366 residues with Ala. The mutant PC synthase had a lower PC synthesis ability than the wild-type enzyme. Further, oxidative conditions severely damaged mutant PC synthase whilst the wild-type enzyme suffered less damage, suggesting that the Cys-rich region of PC synthase may play an important role in anti-oxidation activity. Although the C-terminal of PC synthase is not conserved, our studies support the possibility that this region performs several important biological functions. |
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