Reaction of celite-bound fluorescein isothiocyanate with the 50S E. coli ribosomal subunit

The reaction of Celite-bound fluorescein isothiocyanate with E. coli 50S ribosomes and the 50S moiety of the intact 70S particle has been studied. Approximately five dyes react per 50S particle at pH 8.6 or 9.0. Substantial biological activity is retained. No significant difference between the pattern of reactivity of free and complexed 50S particle can be detected. This suggests the absence of major shielding or conformational changes induced in the 50S by combination with the 30S subunit. The most reactive proteins are L1, L2, L3, and L21. Protein L3 is found in 1.5 and 3 m LiCl core particles where it is still very reactive toward fluorescein. Some other core particle proteins are more reactive than they are in the intact ribosome. In general this work supports previous findings that the proteins of the intact 50S subunit are much less exposed than those of the 30S particle.