Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells |
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| Authors: | Email author" target="_blank">Andreas?BlechaEmail author Kristof?Zarschler Klaas?A?Sjollema Marten?Veenhuis Gerhard?R?del |
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| Institution: | 1.Institut für Genetik,Technische Universit?t Dresden,Dresden,Germany;2.Eukaryotic Microbiology,University of Groningen,Groningen Biomolecular Sciences and Biotechnology Institute (GBB),The Netherlands. |
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| Abstract: | Background Native as well as recombinant bacterial cell surface layer (S-layer) protein of Geobacillus (G.) stearothermophilus ATCC 12980 assembles to supramolecular structures with an oblique symmetry. Upon expression in E. coli, S-layer self assembly products are formed in the cytosol. We tested the expression and assembly of a fusion protein, consisting
of the mature part (aa 31–1099) of the S-layer protein and EGFP (enhanced green fluorescent protein), in eukaryotic host cells,
the yeast Saccharomyces cerevisiae and human HeLa cells. |
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