Inhibition of mammalian host cell infection by insect-derived, metacyclic forms of Trypanosoma cruzi in the presence of human or rabbit anti-T. cruzi antibodies |
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| Authors: | J J Wirth F Kierszenbaum |
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| Affiliation: | 1. Key Laboratory of Monitoring and Estimate for Environment and Disaster of Hubei Province, Institute of Geodesy and Geophysics, Chinese Academy of Sciences, Wuhan 430077, China;2. School of Geography, University of Nottingham, NG7 2RD Nottingham, UK;3. State Key Laboratory of Resources and Environmental Information System, Institute of Geographic Sciences & Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China;4. Lancaster Environment Center, Faculty of Science and Technology, Lancaster University, Lancaster LA1 4YQ, UK;5. School of Geography and Environmental Science, University of Southampton, Highfield, Southampton SO17 1BJ, UK;6. School of Natural and Built Environment, Queen''s University, Belfast, Belfast BT7 1NN, UK;1. Pediatric Emergency Department, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain;2. Pediatric Surgery Department, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain;3. Biochemistry Laboratory, Hospital General Universitario Gregorio Marañón, Madrid 28007, Spain |
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| Abstract: | The presence of serum from chronic chagasic patients or rabbits immunized with killed epimastigote forms of Trypanosoma cruzi inhibited infection of rat heart myoblasts by insect-vector (Triatoma infestans)-derived, metacyclic forms of Trypanosoma cruzi. The effect was produced even after diluting the chagasic serum to non-agglutinating levels and was evidenced by marked reductions in both the percentage of infected myoblasts and the number of parasites per 100 cells. Human IgG or IgM purified from chronic chagasic serum and serum from rabbits immunized with killed T. cruzi epimastigotes also reduced both parameters. While previous work has shown that immunological destruction of invasive forms of T. cruzi may underlie the protective effects of the humoral immune response against this parasite, the present in vitro results suggest that specific anti- T. cruzi antibodies could also contribute to protection via inhibition of host cell infection by the vectortransmissible form of the parasite. |
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