The molecular basis of I-R hybrid dysgenesis in Drosophila melanogaster: identification, cloning, and properties of the I factor |
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Authors: | A Bucheton R Paro H M Sang A Pelisson D J Finnegan |
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Affiliation: | 1. Department of Molecular Biology University of Edinburgh King''s Buildings Edinburgh, EH9 3JR, Scotland;6. Laboratoire de Génétique et Biologie Cellulaire Université de Clermont-Ferrand B. P. 45, 68170 Aubière, France |
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Abstract: | We have analyzed two mutations of the white-eye gene, which arose in flies subject to I-R hybrid dysgenesis. These mutations are associated with insertions of apparently identical 5.4 kb sequences, which we have cloned. We believe that these insertions are copies of the I factor controlling I-R hybrid dysgenesis. The I factor is not a member of the copia-like or fold-back classes of transposable elements and has no sequence homology with the P factor that controls P-M dysgenesis. All strains of D. melanogaster contain I-factor sequences. Those present in reactive strains must represent inactive I elements. I elements have a remarkably similar sequence organization in all reactive strains and are located in peri-centromeric regions. Inducer strains appear to contain both I elements, located in peri-centromeric regions, and 10-15 copies of the complete I factor at sites on the chromosome arms. |
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