Activation of pineal and brain acetyl-COA hydrolase by cystamine: an apparent case of disulfide exchange. |
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| Authors: | M A Namboodiri J T Favilla D C Klein |
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| Institution: | Section on Neuroendocrinology, Laboratory of Developmental Neurobiology National Institute of Child Health and Human Development National Institutes of Health Bethesda, MD 20205 USA |
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| Abstract: | Rat pineal acetyl-CoA hydrolase was activated about 5-fold by cystamine treatment (30 mM) at pH 6.8 and 10-fold at pH 8.5. Six other disulfides were found to be ineffective or to produce a small activation. Cystamine activation was not reversed when free cystamine was removed, but was reversed by treatment with DTT. Analysis of other tissues indicated acetyl-CoA hydrolase from rat brain, sheep pineal gland and chicken pineal gland could also be activated by cystamine. In contrast, cystamine activation of rat liver acetyl-CoA hydrolase was not seen. |
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