Chemical and histochemical studies of normal and diseased gastrointestinal tract. IV. A comparison of histochemical and chemical methods for the estimation of side chain O-acylated sialic acids |
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| Authors: | P E Reid C W Ramey D A Owen W L Dunn D A Lazosky and M G Clay |
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| Institution: | (1) Department of Pathology, Faculty of Medicine, University of British Columbia, V6T 1W5 Vancouver, BC, Canada;(2) Department of Surgery, Faculty of Medicine, University of British Columbia, V6T 1W5 Vancouver, BC, Canada |
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| Abstract: | Summary Statistically significant correlations were obtained between a chemical assay for the proportion of colonic epithelial glycoprotein sialic acids with side chainO-acyl substituents and two histochemical methods, the PBT-KOH-PAS sequence (r
s=0.7485 forN=31,P=0.01, one-sided test) and the PAPT-KOH-Bh-PAS procedure (r
s=0.7024 forN=34). A positive correlation (r
s=0.8654 forN=30,P=0.01) was also obtained between the results of the two histochemical procedures. It is concluded that, on average, histochemical observations are a reliable semiquantitative comparative method for the estimation of side chainO-acetylated sialic acids. |
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