In vivo biosynthesis and turnover of 35S-labeled glomerular basement membrane |
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Authors: | Margo Panush Cohen Maria Linda Surma |
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Institution: | Wayne State University School of Medicine, Department of Medicine, 540 East Canfield, Detroit, MI 48201, U.S.A. |
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Abstract: | Renal glomerular basement membrane was labeled in vivo by the injection of tracer amounts of radioactive sulfate into normal adult rats. The biosynthesis and turnover of 35S]glycosaminoglycans in purified basement membrane was determined from the specific activity of 35S in pronase digests of basement membranes isolated 1–7 days after injection. Peak radioactive labeling occurred 24 h after injection following which the specific activity of basement membrane sulfate, expressed as cpm/μg uronic acid, progressively declined over the ensuing period of study. The biologic half-life of radioactive sulfate in basement membrane was estimated at about 7 days, which is within the range previously reported for 35S]glycosaminoglycans in whole renal cortex. The findings indicate that 35S-labeled components of glomerular basement membrane have a relatively rapid turnover. |
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Keywords: | Basement membrane synthesis Glycosaminoglycan (Rat kidney) |
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