Estrogenic regulation of skeletal muscle proteome: a study of premenopausal women and postmenopausal MZ cotwins discordant for hormonal therapy |
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Authors: | Eija K Laakkonen Rabah Soliymani Sira Karvinen Jaakko Kaprio Urho M Kujala Marc Baumann Sarianna Sipilä Vuokko Kovanen Maciej Lalowski |
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Institution: | 1. Faculty of Sport and Health Sciences, Gerontology Research Center, University of Jyv?skyl?, Jyv?skyl?, Finland;2. Medicum, Biochemistry/Developmental Biology, Meilahti Clinical Proteomics Core Facility, University of Helsinki, Helsinki, Finland;3. Divisions of Rehabilitation Science and Physical Therapy, Department of Rehabilitation Medicine, Medical School, University of Minnesota, Minneapolis, MN, USA;4. Institute for Molecular Medicine FIMM, University of Helsinki, Helsinki, Finland;5. Faculty of Sport and Health Sciences, University of Jyv?skyl?, Jyv?skyl?, Finland |
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Abstract: | Female middle age is characterized by a decline in skeletal muscle mass and performance, predisposing women to sarcopenia, functional limitations, and metabolic dysfunction as they age. Menopausal loss of ovarian function leading to low circulating level of 17β‐estradiol has been suggested as a contributing factor to aging‐related muscle deterioration. However, the underlying molecular mechanisms remain largely unknown and thus far androgens have been considered as a major anabolic hormone for skeletal muscle. We utilized muscle samples from 24 pre‐ and postmenopausal women to establish proteome‐wide profiles, associated with the difference in age (30–34 years old vs. 54–62 years old), menopausal status (premenopausal vs. postmenopausal), and use of hormone replacement therapy (HRT; user vs. nonuser). None of the premenopausal women used hormonal medication while the postmenopausal women were monozygotic (MZ) cotwin pairs of whom the other sister was current HRT user or the other had never used HRT. Label‐free proteomic analyses resulted in the quantification of 797 muscle proteins of which 145 proteins were for the first time associated with female aging using proteomics. Furthermore, we identified 17β‐estradiol as a potential upstream regulator of the observed differences in muscle energy pathways. These findings pinpoint the underlying molecular mechanisms of the metabolic dysfunction accruing upon menopause, thus having implications for understanding the complex functional interactions between female reproductive hormones and health. |
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Keywords: | estrogenic regulation female muscle functional annotation hormone replacement therapy label‐free protein quantitation nano‐LC‐HD‐MSE |
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