Hydrogen peroxide release from human blood platelets |
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| Authors: | Alessandro Finazzi-Agrò Adriana Menichelli Mauro Persiani Giuseppino Biancini Domenico Del Principe |
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| Institution: | 1. Institute of Biochemistry, University of Rome, and CNR Center of Molecular Biology, Rome, Italy;2. First Department of Pediatrics, V. le Regina Elena, Medical School, University of Rome, 324, 00161, Rome, Italy |
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| Abstract: | The release of hydrogen peroxide from human blood platelets after stimulation with particulate membrane-perturbing agents has been determined by fluorescence using scopoletin as the detecting agent. Platelet suspensions containing less than 1 polymorphonuclear leukocyte/108 platelets showed a significant release of hydrogen peroxide (6.11 nmol/109 platelets per 20 min, S.D., 0.26, n=9) after addition of zymosan or latex particles, compared to unstimulated platelets. The release of hydrogen peroxide was only observed when the scopoletin was added to the platelet suspensions during the stimulation. Any attempt to determine hydrogen peroxide release in the supernatant at the end of the incubation with zymosan or latex failed. A NADH-dependent production of hydrogen peroxide was observed by measuring the difference of oxygen uptake in the presence and absence of catalase (500 units), which was not inhibited by potassium cyanide (1 mM). By this method the NADH-dependent cyanide-insensitive peroxide production and release was 6.0 nmol/109 platelets per 20 min from resting platelets (S.D., 2, n=6) vs. 15 nmol/109 platelets per 20 min from stimulated platelets (S.D., 2, n=6). |
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| Keywords: | Hydrogen peroxide release NADH oxidase (Human platelet) |
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