Protective effects of taurine against oxidative stress in the heart of MsrA knockout mice |
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Authors: | Chen G Nan C Tian Jie Jean-Charles P Li Y Weissbach H Huang X P |
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Institution: | Division of Cardiology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China; Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, Florida 33431. |
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Abstract: | Taurine has been shown to have potent anti‐oxidant properties under various pathophysiological conditions. We reported previously a cellular dysfunction and mitochondrial damage in cardiac myocytes of methionine sulfoxide reductase A (MsrA) gene knockout mice (MsrA?/?). In the present study, we have explored the protective effects of taurine against oxidative stress in the heart of MsrA?/? mice with or without taurine treatment. Cardiac cell contractility and Ca2+ dynamics were measured using cell‐based assays and in vivo cardiac function was monitored using high‐resolution echocardiography in the tested animals. Our data have shown that MsrA?/? mice exhibited a progressive cardiac dysfunction with a significant decrease of ejection fraction (EF) and fraction shortening (FS) at age of 8 months compared to the wild type controls at the same age. However, the dysfunction was corrected in MsrA?/? mice treated with taurine supplement in the diet for 5 months. We further investigated the cellular mechanism underlying the protective effect of taurine in the heart. Our data indicated that cardiac myocytes from MsrA?/? mice treated with taurine exhibited an improved cell contraction and could tolerate oxidative stress better. Furthermore, taurine treatment reduced significantly the protein oxidation levels in mitochondria of MsrA?/? hearts, suggesting an anti‐oxidant effect of taurine in cardiac mitochondria. Our study demonstrates that long‐term treatment of taurine as a diet supplement is beneficial to a heart that is vulnerable to environmental oxidative stresses. J. Cell. Biochem. 113: 3559–3566, 2012. © 2012 Wiley Periodicals, Inc. |
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Keywords: | TAURINE DIET SUPPLEMENT HEART MITOCHONDRIA ANTI‐OXIDANT |
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