Biosynthesis of coumarin glycosides by transgenic hairy roots of Polygonum multiflorum |
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Authors: | Zhou Liangbin Tian Tian Xue Bailin Song Liyan Liu Ling Yu Rongmin |
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Institution: | Department of Natural Products Chemistry, Jinan University, Guangzhou, China. |
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Abstract: | To investigate the substrate specificity and regio-selectivity of coumarin glycosyltransferases in transgenic hairy roots of Polygonum multiflorum, esculetin (1) and eight hydroxycoumarins (2-9) were employed as substrates. Nine corresponding glycosides (10-18) involving four new compounds, 6-chloro-4-methylcoumarin 7-O-β-D-glucopyranoside (15), 6-chloro-4-phenylcoumarin 7-O-β-D-glucopyranoside (16), 8-hydroxy-4-methylcoumarin 7-O-β-D-glucopyranoside (17), and 8-allyl-4-methylcoumarin 7-O-β-D-glucopyranoside (18), were biosynthesized by the hairy roots. |
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