The CSC connects three major axonemal complexes involved in dynein regulation |
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Authors: | Heuser Thomas Dymek Erin E Lin Jianfeng Smith Elizabeth F Nicastro Daniela |
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Affiliation: | Biology Department, Rosenstiel Center, Brandeis University, Waltham, MA 02454 Department of Biological Sciences, Dartmouth College, Hanover, NH 03755. |
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Abstract: | Motile cilia and flagella are highly conserved organelles that play important roles in human health and development. We recently discovered a calmodulin- and spoke-associ-ated complex (CSC) that is required for wild-type motility and for the stable assembly of a subset of radial spokes. Using cryo-electron tomography, we present the first structure-based localization model of the CSC. Chlamydomonas flagella have two full-length radial spokes, RS1 and RS2, and a shorter RS3 homologue, the RS3 stand-in (RS3S). Using newly developed techniques for analyzing samples with structural heterogeneity, we demonstrate that the CSC connects three major axonemal complexes involved in dynein regulation: RS2, the nexin-dynein regulatory complex (N-DRC), and RS3S. These results provide insights into how signals from the radial spokes may be transmitted to the N-DRC and ultimately to the dynein motors. Our results also indicate that although structurally very similar, RS1 and RS2 likely serve different functions in regulating flagellar motility. |
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