Change in mutagenic activity of genistein after a nitrite treatment |
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Authors: | Masuda Shuichi Shimamura Yuko Kato Tatsuya Yu-Feng Tan Iwamoto Ken-ichi Kinae Naohide |
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Affiliation: | School of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences and Global COE Program, University of Shizuoka, Japan. masudas@u-shizuoka-ken.ac.jp |
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Abstract: | This study examined the mutagenic activity of genistein after a nitrite treatment under acidic conditions. Nitrite-treated genistein exhibited mutagenic activity toward Salmonella typhimurium strains TA 100 and TA 98 with or without S9 mix. Nitrite-treated genistein was demonstrated by electron spin resonance to generate radicals. An instrumental analysis showed 3'-nitro-genistein to have been formed in the reaction mixture. However, 3'-nitro-genistein did not exhibit mutagenic activity toward the S. typhimurium strains, suggesting that other mutagens might also have been formed in the reaction mixture. The clastogenic properties of nitrite-treated genistein and 3'-nitro-genistein were examined by a micronucleus test with male ICR mice. Nitrite-treated genistein and 3'-nitro-genistein showed a significantly higher frequency of micronucleated reticulocytes in mice than in the control group. These results suggest that a daily oral intake of genistein and nitrite through foodstuffs might induce the formation of various mutagenic compounds in the body. |
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