NMR studies on the interaction between (-)-epigallocatechin gallate and cyclodextrins, free and bonded to silica gels |
| |
| Authors: | Xu Jun Tan Tianwei Janson Jan-Christer Kenne Lennart Sandström Corine |
| |
| Institution: | College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. |
| |
| Abstract: | The interaction between (-)-epigallocatechin-3-gallate (EGCG) and beta- or gamma-cyclodextrin (CD), in free solution and bonded to silica beads, has been studied by (1)H HR-MAS NMR spectroscopy. The chromatographic retardation of EGCG on columns packed with CD-silica beads was shown to be due to the interaction of EGCG with the CD ligands because no nonspecific interaction with the silica gel could be observed. EGCG forms a tighter complex with beta-CD than with gamma-CD and NMR data obtained from hydroxy protons together with MM2 calculations suggest that for beta-CD intermolecular hydrogen bonding, in addition to hydrophobic interaction, stabilizes the complex. |
| |
| Keywords: | Cyclodextrins Epigallocatechin gallate HR-MAS NMR Silica beads |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
