| 脯氨酸在小麦愈伤组织培养中的作用初探 |
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| 引用本文： | 陈克贵 朱庆麟. 脯氨酸在小麦愈伤组织培养中的作用初探[J]. 武汉植物学研究, 1993, 11(1): 67-72 |
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| 作者姓名： | 陈克贵 朱庆麟 |
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| 作者单位： | 四川省农科院生物技术核技术研究所，西北农业大学农学系 成都 610066，陕西杨陵 712100 |
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| 摘 要： | 本文初步研究了脯氨酸在小麦愈伤组织培养中的作用。结果初步表明,小麦愈伤组织能主动吸收培养基中的脯氨酸,并在组织内累积、转化;并且,脯氨酸能刺激小麦愈伤组织苯丙氨酸氨解酶活性,促进苯丙烷类代谢,在愈伤组织中形成导管系统,有利于长期继代培养。
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| 关 键 词： | 小麦 愈伤组织培养 脯氨酸 游离氨基酸 苯丙氨酸氨解酶 |
| 收稿时间： | 1991-06-05 |
| 修稿时间： | 1992-07-01 |
A PRELIMINARY STUDY ON THE L-PROLINE ROLES IN WHEAT CALLUS CULTURE |
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| Chen Kegui. A PRELIMINARY STUDY ON THE L-PROLINE ROLES IN WHEAT CALLUS CULTURE[J]. Journal of Wuhan Botanical Research, 1993, 11(1): 67-72 |
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| Authors: | Chen Kegui |
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| Affiliation: | 1. Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu 610066;2. Department of Agronomy, Northwestern Agricultural University, Yangling, Shaanxi 712100 |
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| Abstract: | Analysis of free amino acids shows that the wheat callus can actively absorbe the L-proline in the medium and accumlate it in vivo, and also transform it into other amino acids. The formation of tracheary system in the long term subculture of wheat callus is due to the increase of phenlpropanoid metabolism according to the results of increase of phenylalanine ammonia lyase(PAL)activity. In this paper the roles of L-proline in wheat callus culture are discussed on the results of long-term subculture growth on the media added other several amino acids and relevant reports in this aspect. |
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| Keywords: | Triticum aestivum L. Callus L-proline Free amino aeids Phenylalanine ammonia lyase (PAL) |
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