Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate. |
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| Authors: | T R Hawkes T Lewis J R Coggins D M Mousdale D J Lowe and R N Thorneley |
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| Institution: | I.C.I. Agrochemical, Jcalotts Hill Research Station, Bracknell, Berks, U.K. |
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| Abstract: | The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state 'burst' or 'lag' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed. |
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