Specific characteristics of the strains isolated from a thermoacidophilic microbial community oxidizing antimony sulfide ore |
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Authors: | A E Zhuravleva I A Tsaplina T F Kondrat’eva |
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Institution: | 1.Winogradsky Institute of Microbiology,Russian Academy of Sciences,Moscow,Russia |
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Abstract: | Investigation of the phenotypic properties of three mixotrophic bacteria, strains Sb-K, Sb-F, and Sb-S, isolated from an aboriginal
thermoacidophilic microbial community participating in biooxidation of ore with high antimony content (26%) and ore concentrates
from the Olympiadinskoe deposit under semicontinuous cultivation conditions at 46 ± 1°C, revealed the differentiating characteristics
of these strains. The isolated cultures grew lithotrophically through different numbers of transfers: strains Sb-F and Sb-K
grew through seven and eight transfers, respectively, and strain Sb-S grew through two or three transfers. Strains Sb-K and
Sb-S utilized a wide range of organic substrates for active organotrophic growth during nine or ten transfers, while strain
Sb-F was less tolerant to organic compounds. Strain Sb-K grew on a medium with the ore and sulfide ore concentrates in the
pH range of 1.0–3.0. Growth of strains Sb-F and Sb-S occurred in the pH ranges of 1.0–2.5 and 1.5–5.5 on media with Fe2+ and S0, respectively.. The optimal initial pH values of the media, corresponding to the maximum specific growth rates, were 1.6–1.7,
1.9, and 2.0–3.0 for strains Sb-K, Sb-F, and Sb-S, respectively. All three strains were able to grow within a broad temperature
range, 20–65°C, with an optimum at 46°C (Sb-K), 40–46°C (Sb-F), and 48–50°C (Sb-S). According to the results of DNA-DNA hybridization
and phylogenetic analysis, as well as their phenotypic characteristics, the isolates can be classified as novel strains of
species of the genus Sulfobacillus. Strains Sb-K, Sb-F, and Sb-S, isolated as predominant cultures on the media with sulfide compounds, iron, or sulfur, respectively,
were affiliated to the species S. thermotolerans, S. sibiricus, and S. thermosulfidooxidans. |
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