Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth) |
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Authors: | V Ivandic U Walther A Graner |
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Institution: | (1) Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany, DE;(2) Federal Centre for Breeding Research on Cultivated Plants, Institute for Epidemiology and Resistance, 06499 Aschersleben, Germany, DE |
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Abstract: | A dominant gene conferring resistance to all known races of Puccinia hordei Otth was identified in two accessions of Hordeum vulgare ssp. spontaneum. Using restriction fragment length polymorphism (RFLP) markers the gene was mapped on chromosome 2HS in doubled-haploid populations
derived from crosses of both accessions to the susceptible cultivar L94. Until now, complete leaf rust resistance was not
known to be conditioned by genetic factors on this barley chromosome. Therefore, the designation Rph16 is proposed for the gene described in this study. A series of sequence tagged site (STS) and cleaved amplified polymorphic
sequence (CAPS) markers were generated by conversion of RFLP probes which originate from the chromosomal region carrying the
resistance gene. Two PCR-based markers were shown to co-segregate with the Rph16 gene in both populations thus providing the basis for marker-assisted selection.
Received: 20 May 1998 / Accepted: 9 June 1998 |
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Keywords: | CAPS Disease resistance Hordeum vulgare ssp spontaneum RFLP-mapping STS |
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