Concentration of bovine plasmalogen using phospholipase A1 in two-phase system |
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| Authors: | Jeong Kyun Kim Guk Hoon Chung Joon Shick Rhee |
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| Institution: | (1) Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, 305-701, Korea;(2) Doosan Venture BG Biotech, Yongin-Gun, Kyonggi-Do, 449-840, Korea |
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| Abstract: | Plasmalogen that contains choline was concentrated from bovine heart to 95% purity from an initial value of 45%, and ethanolamine-containing plasmalogen from bovine brain to 99% purity from initial 64% using phospholipase A1 from Serratia sp. MK1 in a two-phase system. In the two-phase system, Ca2+ was not required and Tris/HCl buffer and butyl acetate were used as an aqueous phase and a solvent phase of the two-phase system, respectively. |
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| Keywords: | concentration emulsion system hydrolysis phospholipase A1 plasmalogen Serratia sp MK1 two-phase system |
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