A Modification of the Adenosine Triphosphatase Method to Demonstrate Epidermal Langerhans Cells |
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Authors: | P. G. Robins D. R. Brandon |
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Affiliation: | a Lilly Research Centre, Erl Wood Manor, Windlesham, Surrey, United Kingdom |
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Abstract: | Epidermal Langerhans cells are most commonly demonstrated by utilizing their adenosine triphosphatase reactivity. Although this is one of the best methods available to the light microscopist, it is a capricious technique which does not always permit optimal demonstration of this ceil population. Prolonged fixation in cacodylate formalin and incubation in 1.32 × 10-3 molar adenosine triphosphatase permits reliable reproduction and good definition of these dendritic cells. |
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