几种匍匐翦股颖成熟胚愈伤组织诱导及植株再生

利用成熟种子作为外植体,分析了2,4-D对匍匐翦股颖胚性愈伤组织诱导与植株再生体系的影响,并对体细胞胚的发生过程进行了观察.实验结果表明,在2.0 mg/L 2,4-D 0.1 mg/L 6-BA时胚性愈伤组织的诱导频率最高.随着2,4-D浓度的增加,愈伤组织的诱导和分化能力明显下降.在再生过程中采用1.0 mg/L的6-BA达到了比较好的效果,愈伤组织的再生频率大部分在90%以上.同时发现适当提高肌醇浓度可以使苗长得较为粗壮.在实验中发现匍匐翦股颖的体细胞胚发生过程为球形胚、心形胚、鱼雷胚和子叶胚.

Callus Induction and Plantlet Regeneration from Mature Embryos of Several Creeping Bentgrasses (Agrostis stolonifera)

Mature seeds of creeping bent grass were used to study the effects of 2,4-D on embryonic callus induction and plantlet regeneration of creeping bentgrass and observe somatic embryogenesis of creeping bent-grass.The experiment results showed that the callus induction frequency reached the highest with 2,4-D at 2.0 mg/L and 6-BA at 0.1 mg/L.As the 2,4-D concentrations increased the callus-inducting capacity and callus-differentiating capacity remarkably declined.With 6-BA at 1.0 mg/L,better plantlet regeneration occurred and the plantlet regeneration frequency was more than 90% in most cases.Meanwhile,a properly-increased myoinositol concentration was helpful for the shoots to grow thick and strong.It was also found in the experiment that in creeping bentgrass somatic embryogenesis experienced globular embryoid,heart-shaped embryoid,torpedo-shaped embryoid and cotyledon-shaped embryoid.