A chemiluminescence assay specific for the microsomal metabolite, benzo[a]pyrene-7,8-dihydrodiol |
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| Authors: | Ambler Thompson H H Seliger G H Posner |
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| Institution: | 1. Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218 USA;2. Department of Chemistry, The Johns Hopkins University, Baltimore, Maryland 21218 USA |
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| Abstract: | It is possible to assay for trans-7,8-dihydroxy 7,8-dihydrobenzoa]-pyrene (BP-7,8-dihydrodiol) in complex metabolite mixtures produced during microsomal metabolism of benzoa]pyrene (BP) because only the BP-7,8-dihydrodiol metabolite will produce significant chemiluminescence (CL) in the NaOCl-H2O2 singlet oxygen-generating system. The limiting CL sensitivity is 30 pmol in a 1-ml CL reaction mixture. CL assays for BP-7,8-dihydrodiol in microsomal reaction solutions gave concentrations identical with those determined by calibrated high-performance liquid chromatography. |
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