A cell attachment assay for use in the standardization of serum products |
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Authors: | D J Giard |
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Institution: | Cell Culture Center, Massachusetts Institute of Technology, Cambridge 02139. |
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Abstract: | A culture tube assay has been developed which can be used to measure relative amounts of cell attachment activity present in different batches of serum or serum products. The assay utilizes a transformed line of BHK-21 cells which is highly dependent upon serum factors for attachment when the cells are subjected to mild liquid shear forces (tube rotation of 1 rpm). Approximately fivefold differences in attachment activity were observed between different batches of bovine calf serum, while up to 20-fold differences were observed between different batches of horse serum. Less than twofold differences were seen between different lots of fetal bovine serum. The assay appears to have application as a quality control measure for screening serum products. |
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