Lipopolysaccharide in the outer membrane of the filamentous prochlorophyte Prochlorothrix hollandica |
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Authors: | Uwe J Jürgens |
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Institution: | Institut für Biologie II, Mikrobiologie, Albert-Ludwigs-Universität, Freiburg i. Br., F.R.G. |
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Abstract: | Abstract A lipopolysaccharide (LPS) fraction was isolated from Prochlorothrix hollandica by hot phenol/water extraction. Negatively stained preparations of an aqueous LPS dispersion showed the triple-layered appearance of the LPS aggregates. Glucose (main sugar), rhamnose, fucose, galactose, mannose, xylose, and 3- O -methyl-xylose were found as the constituents of the polysaccharide moiety. Glucosamine and the 3-hydroxy fatty acids, 3-OH-16:0, 3-OH-14:0, and the rarely detected iso-3-OH-15:0, constitute the lipid A of the LPS. l -glycero- d -manno-heptose and 3-deoxy- d -manno-2-octulosonic acid (dOclA), typical components of inner core oligosaccharides from enterobacterial LPS, were lacking in the isolated LPS fraction from Prochlorothrix hollandica . |
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Keywords: | Lipid A Lipopolysaccharide Outer membrane Prochlorophyta Prochlorothrix hollandica Sugar O-methyl ether |
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