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| 激素对转基因雪莲毛状根植株再生及类黄酮产生的影响 | |
| 引用本文： | 乔献丽,蒋曙光,吕晓芬,李凤霞,赵德修. 激素对转基因雪莲毛状根植株再生及类黄酮产生的影响[J]. 生物工程学报, 2011, 27(1): 69-75 |
| 作者姓名： | 乔献丽 蒋曙光 吕晓芬 李凤霞 赵德修 |
| 作者单位： | 1. 中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京100093;中国科学院研究生院,北京100049 2. 河南中医学院第一附属医院,郑州,450000 3. 中国科学院植物研究所光合作用与环境分子生理学重点实验室,北京,100093 |
| 基金项目： | 国家自然科学基金 (No. 30472158) 资助。 |
| 摘 要： | 为了研究外源激素GA3和IAA对3个转基因新疆雪莲类黄酮高产毛状根系C17、C27、C46的植株再生及其总黄酮含量的影响,在培养基中添加不同浓度的GA3和IAA,结果发现,GA3浓度高于1.0 mg/L时,可诱导毛状根系产生不定芽,其中以GA3浓度为2.0 mg/L时,转基因毛状根系C17的不定芽再生率最高,可达82％。高压液相色谱以及紫外分光光度法测定结果表明,与未用激素处理的毛状根和它的再生植株相比,外源激素GA3和IAA能显著提高毛状根培养物中芹菜素和总黄酮的含量。毛状根系的组织干重与类黄酮的含量没有相关性,但毛状根系的再生率与类黄酮的含量几乎呈反相关性。 |
| 关 键 词： | 新疆雪莲 植物激素 植株再生 毛状根 类黄酮 |
| 收稿时间： | 2010-03-18 |
| 修稿时间： | 2010-06-22 |
Effects of phytohormones on plant regeneration and production of flavonoids in transgenic Saussurea involucrata hairy roots |
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| Qiao Xianli,Jiang Shuguang,Li Xiaofeng,Li Fengxia,Zhao Dexiu. Effects of phytohormones on plant regeneration and production of flavonoids in transgenic Saussurea involucrata hairy roots[J]. Chinese journal of biotechnology, 2011, 27(1): 69-75 | |
| Authors: | Qiao Xianli Jiang Shuguang Li Xiaofeng Li Fengxia Zhao Dexiu |
| Affiliation: | Key Laboratory for Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; Graduate School of Chinese Academy of Sciences, Beijing 100049, China;The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China;Key Laboratory for Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; Graduate School of Chinese Academy of Sciences, Beijing 100049, China;Key Laboratory for Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; Graduate School of Chinese Academy of Sciences, Beijing 100049, China;Key Laboratory for Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
| Abstract: | We investigated the plant regeneration and production of flavonoids in three high-yield flavonods transgenic Saussurea involucrata hairy roots C17, C27 and C46 by quantification of two phytohormones GA3 and IAA. The results showed that GA3 concentration at more than 1.0 mg/L could induce adventitious shoots in the hairy root lines. The highest shoot regeneration rate, about 82%, was obtained when the hairy roots C17 were cultured with 2.0 mg/L GA3. The results on HPLC and UV spectrophotometry showed that exogenous application of both GA3 and IAA increased the content of flavonoids in the hairy roots. The contents of flavonoids and apigenin in the hormone-treated hariy roots and regenerates were higher comparing with those in the untreated hairy roots and the regenerates. However, the content of flavonoids was not related to tissue weight, and was negatively related to the regeneration efficiency. |
| Keywords: | Saussurea involucrata phytohormones plant regeneration hairy root flavonoids |
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