|
|||||
|
|
Brain peptide reverses effect of morphine on human lymphocytes |
|
| Authors: | John H Strimas David S Chi and Abba J Kastin |
| Institution: | * Section of Allergy/Immunology, Department of Pediatrics Louisiana State University Medical Center, New Orleans, LA, USA † Division of Allergy/Immunology, Department of Medicine Quillen-Dishner College of Medicine, Johnson City, TN, USA ‡ Endocrinology Section of the Medical Service V.A. Medical Center, University of New Orleans and Tulane University School of Medicine, New Orleans, LA, USA |
| Abstract: | E-rosette formation by human lymphocytes incubated with sheep red blood cells (sRBC) is inhibited by morphine. We studied the ability of the opiate antagonists naloxone and Tyr-MIF-1 (Tyr-Pro-Leu-Gly-NH2) to block this action. Active E-rosette formation by lymphocytes incubated with morphine was reduced from the control of 35.7±1.7% to 23.7±1.5% (p<0.001). Similarly, total E-rosette formation was reduced by morphine from the control of 65.8±1.3% to 53.2±2.9% (p<0.001). These effects were blocked by co-incubation of the lymphocytes with either Tyr-MIF-1 or naloxone (p<0.05). Tyr-MIF-1 was active (p<0.05) at concentrations as dilute as 10−13M. These results indicate that the neuropeptide Tyr-MIF-1 exerts an antiopiate effect at the human T-lymphocyte. |
| Keywords: | Tyr-MIF-1 T-lymphocyte E-rosette Antiopiate Peptide Morphine Naloxone |
| 本文献已被 ScienceDirect 等数据库收录！ | |