Ethanol production from concentrated food waste hydrolysates with yeast cells immobilized on corn stalk |
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Authors: | Yan Shoubao Chen Xiangsong Wu Jingyong Wang Pingchao |
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Institution: | (1) School of Life Science, Huainan Normal University, Huainan, Anhui, 232001, People’s Republic of China;(2) Key Laboratory of Ion Beam Bio-engineering of Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, 230031, People’s Republic of China |
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Abstract: | The aim of the present study was to examine ethanol production from concentrated food waste hydrolysates using whole cells
of S. cerevisiae immobilized on corn stalks. In order to improve cell immobilization efficiency, biological modification of the carrier was
carried out by cellulase hydrolysis. The results show that proper modification of the carrier with cellulase hydrolysis was
suitable for cell immobilization. The mechanism proposed, cellulase hydrolysis, not only increased the immobilized cell concentration,
but also disrupted the sleek surface to become rough and porous, which enhanced ethanol production. In batch fermentation
with an initial reducing sugar concentration of 202.64 ± 1.86 g/l, an optimal ethanol concentration of 87.91 ± 1.98 g/l was
obtained using a modified corn stalk-immobilized cell system. The ethanol concentration produced by the immobilized cells
was 6.9% higher than that produced by the free cells. Ethanol production in the 14th cycle repeated batch fermentation demonstrated
the enhanced stability of the immobilized yeast cells. Under continuous fermentation in an immobilized cell reactor, the maximum
ethanol concentration of 84.85 g/l, and the highest ethanol yield of 0.43 g/g (of reducing sugar) were achieved at hydraulic
retention time (HRT) of 3.10 h, whereas the maximum volumetric ethanol productivity of 43.54 g/l/h was observed at a HRT of
1.55 h. |
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