Characterization of novel diesel-degrading strains <Emphasis Type="Italic">Acinetobacter haemolyticus</Emphasis> MJ01 and <Emphasis Type="Italic">Acinetobacter johnsonii</Emphasis> MJ4 isolated from oil-contaminated soil |
| |
Authors: | Myungjin Lee Sung-Geun Woo Leonid N Ten |
| |
Institution: | (1) Research and Development Division, H-Plus Eco Ltd., BVC 301, KRIBB, Eoeun-dong, Yuseong-gu, Daejeon, 305-333, Republic of Korea;(2) School of Civil and Environmental Engineering, Yonsei University, Seoul, 120-749, Republic of Korea;(3) Department of Biology and Medicinal Science, Pai Chai University, 14 Yeon-Ja 1 Gil, Seo-Gu, Daejeon, 302-735, Republic of Korea |
| |
Abstract: | The diesel-degrading strains, designated as MJ01 and MJ4, were isolated from oil-contaminated soil in Daejeon (South Korea)
and were taxonomically characterized using a polyphasic approach and their diesel oil degradation abilities were analyzed.
The isolates MJ01 and MJ4 were identified as Acinetobacter haemolyticus and Acinetobacter johnsonii, respectively, based on their 16S rDNA gene sequences, DNA–DNA relatedness, fatty acid profiles and various physiological
characteristics. Strains MJ01 and MJ4 were able to use diesel oil as the sole carbon and energy source. Both strains could
degrade over 90% of diesel oil with an initial concentration of 20,000 mg/l after incubation for 7 days, the most significant
degradation occurred during the first 3 days. To our knowledge, this is the first report on diesel oil-degrading microorganisms
among bacterial strains belonging to A. haemolyticus and A. johnsonii. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|