Storekeeper defines a new class of plant-specific DNA-binding proteins and is a putative regulator of patatin expression |
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Authors: | Zourelidou Melina de Torres-Zabala Marta Smith Caroline Bevan Michael W |
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Affiliation: | Department of Cell and Developmental Biology, John Innes Centre, Norwich NR4 7UH, UK. |
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Abstract: | The expression of class I patatin genes is restricted to potato tubers but can be induced in other tissues by exogenous sucrose. Here we show that tuber-specific and sucrose-inducible gene expression is reduced in transgenic potato plants by mutations in a conserved 10 base pair motif within the B-box of the patatin promoter. In a southwestern screen, we have isolated a novel DNA-binding protein designated Storekeeper (STK) that specifically recognises the B-box motif in vitro. Gel shift experiments with an STK-specific antibody suggest that STK is the B-box binding protein found in tuber nuclei. We propose that STK, the defining member of a new class of DNA binding proteins, regulates patatin expression in potato tubers via the B-box motif. |
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