Preparation and characterization of polyclonal and monoclonal antibodies against the insecticide DDT |
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Authors: | D Bürgisser S Frey B Gutte S Klauser |
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Affiliation: | Biochemisches Institut der Universit?t Zürich, Switzerland. |
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Abstract: | A synthetic DDT derivative in which the molecular structure of DDT was completely retained was coupled to bovine serum albumin. Animals were immunized with the DDT-bovine serum albumin conjugate and polyclonal and monoclonal antibodies against the insecticide were isolated. These antibodies seemed to be the first true anti-DDT antibodies and distinguished much better between DDT and DDT metabolites than previously prepared anti-DDT antisera. In competitive solid phase radioimmunoassays, DDT concentrations as low as 10 nM or 0.0035 mg/1 were detectable. The anti-DDT antibodies can be used for environmental analyses and lend themselves to the elucidation of the structure of the DDT binding site. |
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