抗氧化剂对皮肤角质细胞体外寿命的影响

考察了抗氧化剂对鼠角质细胞体外培养寿命的影响。实验发现在角质细胞的体外培养过程中添加抗氧化剂有利于延长细胞的寿命，其中效果最好的是巯基乙醇，其次为过氧化氢酶和SOD，但在体外培养过程中，角质细胞生长速率仍然逐渐下降。实验还发现，添加抗氧化剂可在一定程度上提高角质细胞的克隆形成率，减缓细胞衰老速率。同时，通过考察鼠表皮角质细胞衰老动力学，获得了对应于不同抗氧化剂的细胞衰老动力学常数。 

The Effect of Antioxidants on the in vitro Life-span of Keratinocyte

The effect of antioxidants on the in vitro life span of mouse keratinocytes was investigated in this work. It was found that the life span of the keratinocytes cultured in the medium supplemented with antioxidants was extended significantly. The most beneficial antioxidant used in this work was the mercaptoethanol, followed by the catalase and SOD. However, the growth rates of keratinocytes in vitro under all the experimental conditions still declined with the culture time. It was also found that the antioxidants added in the medium were also helpful to enhance the keratinocyte colony formation. In addition, the aging kinetics of the mouse epidermal keratinocytes in vitro were analyzed, and finally the aging rate constants corresponding to antioxidants used were calculated.