蛋白质组学技术及其在生物医学上的应用

蛋白质组学部分承用了创立于二十多年前的二维电泳技术。基于其高分辩能力 ,二维电泳主要用于分离和检测复杂混合物中的蛋白质。虽然没有获得更多的改进 ,但是二维电泳结合了通过质谱测定蛋白质的最新进展而成为蛋白质组学中的一项重要技术。随着人类基因组计划项目的完成及由此而产生的大量基因数据库和使用这些数据的生物信息技术 ,科学家们的下一个目标是解析生物体的完整蛋白质组 ,把蛋白质组学数据与基因组学数据关联起来并有机地结合而成为一项有力的工具以阐明病理学中的蛋白质功能、衰老的过程及发现新药目标蛋白质和疾病标识物等。文章综述了蛋白质组学技术的最新知识及其在生物医学研究中的潜在应用

Proteomic Technology and Its Biomedical Applications

Proteomics has its origins in two dimensional gel electrophoresis (2 DE), a technique developed more than twenty years ago. 2 DE has a high resolution capacity, and was initially used primarily for separating and characterizing proteins in complex mixtures. 2 DE remains an important tool for protein identification, but is now normally coupled with mass spectrometry (MS), a technique which has advanced considerably in recent years. The recent completion of human genome project has produced a large DNA database which can be utilized through bioinformatics, and the next challenge for scientists is to uncover the entire proteome of a particular organism. The integration of genomic and proteomic data will help to elucidate the functions of proteins in the pathogenesis of diseases and the ageing process, and could lead to the discovery of novel drug target proteins and biomarkers of diseases. This review describes recent advances in proteomic technology and discusses the potential applications of proteomics in biomedical research.