Purification of beta-glucosidase activities from bovine spleen by affinity chromatography |
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Authors: | J N Kanfer R A Mumford S S Raghavan J Byrd |
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Affiliation: | 1. Eunice Kennedy Shriver Center, Walter E. Fernald State School, Waltham, MA USA 02154;2. Neurology Research, Massachusetts General Hospital, Boston, MA 02114 USA |
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Abstract: | Bovine spleen β-d-glucosidase, glucosylceramide: β-d-glucosidase and glucosylsphingosine: β-d-glucosidase were purified by chromatography on a “gluconate” Sepharose column. Ten other lysosomal acid hydrolases, present in the preparation applied to the column, were absent from the glucosidase fraction. |
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