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Purification of beta-glucosidase activities from bovine spleen by affinity chromatography
Authors:J N Kanfer  R A Mumford  S S Raghavan  J Byrd
Affiliation:1. Eunice Kennedy Shriver Center, Walter E. Fernald State School, Waltham, MA USA 02154;2. Neurology Research, Massachusetts General Hospital, Boston, MA 02114 USA
Abstract:Bovine spleen β-d-glucosidase, glucosylceramide: β-d-glucosidase and glucosylsphingosine: β-d-glucosidase were purified by chromatography on a “gluconate” Sepharose column. Ten other lysosomal acid hydrolases, present in the preparation applied to the column, were absent from the glucosidase fraction.
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